The work aims to prepare a new colorimetric indicator film of red pigment of Herba Peristrophe Japonica (HPJH) combined with water chestnut starch, and study the dynamic response characteristics of the prepared colorimetric indicator film by UV-VIS absorption spectra and gray average values. HPJH was extracted from the plant of Herba Peristrophe Japonica. The colorimetric indicator film was prepared with blending HPJH, water chestnut starch and glycerol. The oxidation resistance of the film could be changed by the introduction of HPJH. Appropriate amount of HPJH could promote the uniformity of film structure. The film formation effect was the best, when the mass fraction of red pigment was 8%, and the DPPH free radical scavenging rate reached 88.70%. The ΔE value was 1.649 7, when film color stability experiment was end. It was more convincing to show the response of colorimetric indicator film to triethylamine in different humidity conditions by the average gray value. HPJH extracted from Herba Peristrophe Japonica can improve the indication effect of the water chestnut starch film. The colorimetric indicator film has strong oxidation resistance and has color change response to triethylamine. It has dynamic response characteristics in monitoring the freshness of pork, fish and lettuce.