The work aims to explore the effects of different harvest periods on the quality of Lonicera caerulea L. after 1-MCP treatment during 0~75 d, so as to provide a reference for the optimal harvest period of Lonicera caerulea L for long-term storage. Lonicera caerulea L. of two harvest periods (harvest period I:45 d after fruit flowering; harvest period II:50 d after fruit flowering) was used as the experimental materials. After treated with 1-methylcyclopropene (1-MCP), the Lonicera caerulea L. was put into a crisper box and stored at (−0.5±0.3) °C for 75 days and then sampled every 15 days to measure organoleptic, nutritional and physiological indicators. Compared with the harvest period II. group, the harvest period I. group was better to maintain the fruit hardness, reducing the phenomenon of fruit rot and mildew, juice and fruit frost. After storage of 75 d, the respiration intensity of the fruit in the harvest period I. group was 202.79 mg/(kg.h) and the ethylene formation rate was 23.27 μL/(kg.h),4.90% and 10.08% lower than those in the harvest period II. group, respectively. The he accumulation of malondialdehyde content and the increase of relative conductivity could be inhibited. At the end of storage, the nutritional qualities of ascorbic acid, anthocyanins, total phenols and soluble protein (P<0.05) were significantly maintained. Lonicera caerulea L. from harvest period I. group (45 d after fruit flowering) can maintain good appearance and intrinsic quality after long-term storage.