The work aims to analyze the impact resistance of apple in transportation packaging environment and optimize the design of apple-EPS foam cushion packaging. The stress-strain curve of apple was obtained by compression test on universal compression tester. Finite element model of apple transportation packaging system was established by ABAQUS/Explicit and analyzed by finite element simulation. Crushable Foam model was used for foamed polystyrene finite element model and linear elasticity model was used for apple. The impact response of apple falling down in vertical and horizontal layouts along the axis was simulated, and the cushioning effect of EPS foam with different thicknesses was analyzed. The elastic modulus of the apple was 2.44 MPa and the yield limit was 0.38 MPa. The simulation analysis showed that EPS foam could effectively reduce the impact damage to the apple during transportation, and under the same conditions, the horizontal layout of the apple along the axis was more reasonable. By comparison to the numerical analysis method, it can be concluded that the research can provide data support and reference for related fruit and vegetable transportation based on experiment and finite element analysis.