The work aims to study the vibration characteristics of stacking packages with different buffer materials in logistics transportation. Buffer materials EPE and EPS were used as research objects. Three-dimensional modeling software SoildWorks and finite element simulation software Workbench were used to establish the finite element model for modal and harmonic response analysis. The vibration response characteristics of each stacking part were analyzed by the combination of sweep vibration experiment and random vibration experiment. The first excitation energy at the resonant frequency of both packages was greater than the second excitation energy. The amplitude of vibration increased with the increase of the number of layers. The excitation energy of the first and second resonance frequencies of EPE packaging accounted for 35.7% and 3%, while the excitation energy of the first and second resonance frequencies of EPS packaging accounted for 26.8% and 16.8%. The resonance region of EPE packaging was mainly distributed in 15-40 Hz, while the resonance frequency region of EPS packaging was distributed in 15-40 Hz and 60-80 Hz. The middle and upper packaging was controlled by the first resonance frequency, the bottom packaging of EPS material was affected by multiple resonance frequencies. And the bottom packaging of EPE material was controlled by the second resonance frequency. The vibration absorbing and buffering performance of EPE under cyclic loading is better than that of EPS. The corresponding anti-vibration performance curve is drawn by comparing the experimental data with the finite element simulation data, and the reliability of the finite element simulation is verified. It provides theoretical guidance for selection of different buffer material combinations for stacking products.