The work aims to provide key technical parameters for strawberries during circulation and distribution, to contribute to high-quality development of e-commerce on strawberries. The physiological and quality characteristics of strawberries packaged by PET box containing a foam tray were analyzed in direct delivery, super market and e-commerce by laboratory model, respectively. Respiration intensity and ethylene production rate of strawberries in e-commerce were significantly higher than that in direct delivery, but had not obvious difference with that in super market. Except that anthocyanin content of strawberries was the highest in e-commerce, other nutritional qualities including total soluble solid, soluble protein, ascorbic acid, total phenol, total antioxidant capacity and titratable acid had not significant difference among the three distribution modes. The results showed that respiration intensity and ethylene production rate of strawberries increased with the increase of distribution time. The nutritional quality did not significantly change during the short distribution time. Under the appropriate condition of buffer packaging at 18-20 ℃, the nutritional quality of strawberries can be well maintained during e-commerce distribution, where the short and slight mechanical vibration at 200 r/min for 15 min has little influence on the physiological characteristics of strawberries.