The work aims to solve the problems of flatness and adhesion in the process of cigarette tipping paper loading and clarify the influencing factors that affect the key index of tipping paper loading applicability. With tipping paper as the research object, the change of tipping paper flatness under different conditions was analyzed by changing and controlling the ambient temperature and humidity. Then, the surface microstructure was investigated by scanning electron microscope, and the corresponding penetration characteristics and wetting performance were studied by liquid penetration method, dynamic penetration method and contact angle method. The difference in the same index for different suppliers was compared and analyzed. When the temperature and humidity were controlled within the range of 20 ℃/50% to 30 ℃/60%, the tipping paper flatness was less than 5 mm, and its own penetration and wetting performance was less affected. The key index of tipping paper loading applicability such as flatness and adhesion is not only affected by the base paper and printing process, but also by the ambient temperature and humidity. Selecting suitable tipping glue for tipping paper produced by different processes according to the penetration and wetting performance can effectively improve the loading applicability and reduce material loss and quality defects.