The work aims to study the effect of cigarette multi-layer packaging on moisture barrier in a dry environment in view of that the barrier performance of cigarette packaging plays a key role in the quality stability of cigarette products. A self-developed testing system, dynamic moisture analysis climate box, was employed to investigate the moisture diffusion process of different packaging materials under specific temperature and humidity conditions, calculate the moisture transmission rate and quantitatively characterize the moisture barrier performance of packaging materials. The evaluation method of moisture barrier performance of multi-layer packaging of cigarette packet could accurately quantify and characterize the moisture barrier performance of the packaging. Under low humidity conditions, the amounts of moisture permeating through the clearances and materials of each layer of the cigarette packaging were different. For Bopp film, more than 60% of the moisture mainly diffused through the material. For different label paper and lining paper, the distribution ratios of moisture diffusing through the material and the clearances were different. For the moisture barrier performance of the overall packaging, the Bopp film played a decisive role in the moisture stability of cigarette, of which the contribution was above 90%. The moisture barrier performance of hard packaging was better than that of the soft packaging. The moisture diffusion rate of cigarette in different packaging was predicted accurately. Under low humidity condition, the moisture diffusion rate of hard-packed cigarette was significantly lower than that of soft-packed cigarette. The moisture barrier test and evaluation method of cigarette multi-layer packaging are established. The moisture barrier performance of each layer and different parts of cigarette packet is quantitatively characterized. Finally, the key factors that affect the moisture stability of cigarette in packet are identified.