The work aims to select and analyze the engineering design parameters of the antifreeze insulation packaging box and its internal refrigerant to improve the antifreeze packaging scheme in view of the different low temperature climates in cold regions and the requirements for insulation time of different temperature-sensitive products at 0~8 ℃. Firstly, the finite element modeling of antifreeze insulation box was carried out to analyze the transient temperature change and effective insulation time in low temperature environment. Then, combined with the actual test, the model credibility was validated. The finite element model was used to simulate the thermal insulation performance of box in low temperature environment. The basic parameters of the insulation box were fixed and the effect of phase change temperature and latent heat of the refrigerant on thermal insulation performance was studied. Then, the basic parameters of the refrigerant were fixed, and the effect of the thickness and thermal conductivity of the box on thermal insulation performance was studied. The temperature control diagram for the insulation time of box was fitted respectively, and the parameter selection of the refrigerant and the box for different insulation time was discussed. Through the comparison between simulation and test, the average deviation of antifreeze insulation box model was 4.6%, meeting the engineering requirements. According to the simulation data, the engineering design parameters of refrigerant and insulation box were reasonable and could meet the requirements of low temperature environment and insulation time. On the premise of meeting external low temperature environment and insulation time requirements of cold chain logistics, scientific and reasonable selection of engineering design parameters of refrigerant and insulation box can improve the temperature control efficiency of the insulation box, which can lay a foundation for the optimal design of antifreeze packaging in cold chain logistics transportation.