The work aims to prepare antifungal nano-preservation materials that can continuously release thymol in response to the demand of the fruit and vegetable industry for nano-controlled release preservation packaging materials, so as to avoid the problem of low preservation efficiency due to the volatility of thymol. Solution blowing spinning (SBS) technology was adopted to prepare poly (caprolactone) (PCL) nanofiber membranes loaded with thymol/2-hydroxy- β-cyclodextrin (THY/HPβCD) envelope, and to evaluate the properties of fiber membranes and the inhibition effect of Botrytis cinerea on the surfa wberries. SBS enables the rapid preparation of THY/HPβCD/PCL nanofiber membranes; the membranes had enhanced water vapor permeability, surface hydrophilicity, thermal stability, sustained and stable thymol release within 72 h, and could inhibit the decay of strawberry fruits inoculated with Botrytis cinerea. The prepared THY/HPβCD/PCL nanofiber membranes have good slow-release and antifungal activities and are expected to be applied to the post-harvest storage and preservation of fruits and vegetables.