Determine the appropriate harvest time of pitaya fruit and explore the changes of fruit quality during storage. With pitaya as the experimental material, the changes of edible quality, physiological and nutritional components of pitaya at different harvest time (July 2, August 28, September 24 and November 12, 2020) after 8 days storage were studied. The fruit harvested in September had the best quality and storability, which was suitable for fresh sale or re-sale after long-term storage. The fruit harvested in July and August had the average quality and storability, which was suitable for direct fresh sale or re-sale after short-term storage and processing. The fruit harvested in November had poor quality and storability, which was suitable for fresh sale and processing. Different harvest time can affect the quality and storage effect of pitaya and the study can provided a theoretical basis for pitaya storage and processing.