The work aims to prepare high oxygen barrier recycled cellulose (RC) films through sol-gel method by dissolving the recycled waste cotton fabrics in LiCl/DMAc solution with water, methanol, ethanol, 50% ethanol solution and acetone as coagulation baths, respectively, so as to improve the value and utilization of waste cotton fabrics. The structure and properties of RC films were investigated with oxygen permeation tester, water vapor transmission tester, tensile tester, infrared spectroscopy and thermogravimetry. The RC films prepared under different coagulation bath conditions all exhibited extremely high oxygen barrier and high tensile strength. Among them, the RC films prepared in acetone coagulation bath had the best oxygen barrier performance, with the oxygen permeability coefficient as low as 2.093 5×10−17 cm3.cm/(cm2.s.Pa), which was 2-4 orders of magnitude lower than the oxygen permeability coefficient of ordinary plastic films under the same test conditions. In addition, the films also had high mechanical properties up to 98 MPa, which was much higher than that of ordinary polyethylene films. This work prepares a degradable RC film with high oxygen barrier and high strength, which provides a new way for recycling and secondary use of waste cotton fabrics.