The work aims to transform the wood packaging waste of Pinus sylvestris into fluorescent carbon quantum dots, so as to explore a new way of realizing the high-value comprehensive utilization of waste wood packaging of Pinus sylvestris. The 60-mesh Pinus sylvestris wood packaging waste was used as the precursor of carbon quantum dots, and sulfuric acid and ethylenediamine were used as sulfur and nitrogen dopants to synthesize Pinus sylvestris-based carbon quantum dots under hydrothermal conditions of 200 ℃ for 10 h. The effects of raw material ratio on the physical structure, chemical structure and optical properties of Pinus sylvestris-based carbon quantum dots were studied by means of UV-Vis spectrophotometer, fluorescence spectrophotometer, transmission electron microscope, Fourier infrared spectrometer and X-ray photoelectron spectrometer. The preparation process of Pinus sylvestris-based carbon quantum dots was optimized with fluorescence quantum yield of carbon dots as the evaluation index. The obtained sulfur nitrogen doped carbon quantum dots were regular spherical, and their average particle size and particle size distribution were 4.30 nm and 2.01-6.63 nm, respectively. When the ratio of raw materials was Pinus sylvestris powder:sulfuric acid:ethylenediamine=1:1:1 (weight ratio), the fluorescence of the doped carbon quantum dots was the strongest and the fluorescence quantum yield was the highest. When the weight ratio of Pinus sylvestris powder, deionized water, sulfuric acid and ethylenediamine was 1.250∶50∶1.250∶1.250, the fluorescence quantum yield of doped carbon quantum dots was about 80 times higher than that of undoped carbon quantum dots. Converting the wood packaging waste of Pinus sylvestris into fluorescent carbon quantum dots is a new way to realize the high-value comprehensive utilization of waste wood packaging of Pinus sylvestris.