The work aims to prepare carbon quantum dots based on waste Loblolly Pine wood packaging to improve the current situation of low comprehensive utilization rate and insufficient functional utilization of waste Loblolly Pine wood packaging, thus realizing high value-added utilization. With waste Loblolly Pine wood packaging as carbon source and urea and phosphoric acid as nitrogen and phosphoric dopants, N-P doped carbon quantum dots were prepared by hydrothermal method at 200 ℃ for 12 h. Then, the physical structure, chemical structure and optical properties of CQDs were characterized by UV-vis spectrophotometer, fluorescence spectrophotometer, transmission electron microscope, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy and the preparation technology realizing high fluorescence intensity and high fluorescence quantum yield was selected. The N-P doped Loblolly Pine CQDs were regular spherical with average particle size of 2.25 nm and particle size distribution of 1.3~3.4 nm. The N-P doped CQDs were uniformly distributed in aqueous solution without obvious aggregation, andwith surface rich in ether bonds, carbon-carbon double bonds, phosphorous and nitrogenous functional groups. When the ratio of Loblolly Pine wood powder:deionized water:urea:phosphoric acid was 1:60:0.5:0.5 (mass ratio), both fluorescence strength and fluorescence quantum yield of the N-P doped Loblolly Pine CQDs were the highest. The fluorescence quantum yield of the N-P doped Loblolly Pine CQDs was 5.54 times higher than that of CQDs without nitrogen and phosphoric acid. It is feasible to prepare fluorescent carbon quantum dots from waste Loblolly Pine wood packaging as carbon source, which provides a new way for high-value and functional utilization of waste wood packaging.