The work aims to explore the effect of sampling direction on tensile fracture behavior of kraft paper with unilateral crack, in order to improve the strength of finished products such as packaging boxes made of kraft paper. First of all, the kraft paper specimens with unilateral cracks in different sampling directions were prepared, and then the relationship graph between different sampling directions and tensile fracture load was measured based on the digital image correlation (DIC) method combined with the micro tension test bed. Next, the micro strain field at the crack tip in different sampling directions was analyzed. Finally, comparative analysis was carried out in combination with the scanning electron microscope test. The results showed that, in general, the kraft paper with unilateral crack still conformed to the law that the longitudinal tensile load of the paper was greater than the transverse tensile load, and the closer the sampling direction to the transverse direction, the greater the strain value in the crack tip area and the smaller the tensile load when fracture occurred. The sampling direction and fiber to fiber bonding strength are two important factors that affect the tensile fracture behavior of kraft paper.