The work aims to investigate the robustness and invisibility of watermarks against different screening frequency and screen angles, so as to transplant digital watermarking technology from the field of digital copyright protection to printing anti-counterfeiting. With the holographic watermarking algorithm in DWT domain as an example, the watermarked image was made into a hologram and the hologram was embedded into the DWT domain of host image in C, M, Y or K layers, to obtain watermarked image. Simulation experiment was carried out to the watermarked image with different screening frequency and screen angles. The robustness and invisibility of watermarked image increased with the increase of the number of screening frequency. The lowest acceptable number of screen frequency was 120 lpi. Under the condition of the same number of screen frequency, the quality of extracted watermarks when the screen angle was 45° were lower than that at 0°, 15° and 75°. The screen angle of 45° is suitable for monochrome printing, but the watermark attack is the most significant. Therefore, the color channel with screen angle of 45° should be avoided as the watermark embedding position.