The work aims to prepare starch film based on mango kernel starch, analyze the film property and provide theoretical guidance and data reference for the application of biodegradable packaging materials. Starch films were prepared with different concentrations of glycerol, carboxymethyl cellulose and gallic acid. The mechanical properties, water vapor transmission coefficient, antioxidant activity and thermal stability of the films were analyzed by modern analytical instruments, and the optimal performance parameters of the films were explored. When the mass fraction of glycerol was 30%, carboxymethyl cellulose was 45% and gallic acid was 4%, the tensile strength, elongation at break, water vapor transmission coefficient and antioxidant activity of the film were 25.28 MPa, 11.66%, 0.14 g.mm/(m2.h.kPa) and 75.93%, respectively. Mango kernel starch can form effective cross-link with additives, which can improve the mechanical property and antioxidant activity of the starch films, reduce the water vapor transmittance coefficient and improve the thermostability of the films.