The work aims to prepare a new chitosan-agarose composite degradable food packaging film to test the mechanical, self-healing and fresh-keeping properties and explore the composite film with the best comprehensive properties. Chitosan and agarose mixed solution was used to prepare food packaging film. Scanning electron microscope, Fourier transform infrared spectrometer, X-ray diffractometer and other characterization means were used to analyze the structure of the packaging film. Universal tensile tester, ultraviolet spectrophotometer, contact angle meter and fresh-keeping experiment were adopted to test the mechanical, optical and fresh-keeping properties of packaging film. Chitosan and agarose interacted to form hydrogen bonds, and the compatibility of the composite film was effectively improved by hydrogen bond self-assembly. The transparency of the prepared degradable food packaging film was as high as 90% and the breaking strength reached 69.1 MPa. In addition, the composite film showed a certain self-healing function. Due to excellent fresh-keeping effect, strawberries could be stored at room temperature of 19 ℃ for up to 10 days, effectively prolonging the shelf life. The composite film had the best comprehensive performance when the mass ratio of chitosan to agarose was 1︰1 in the mixed solution system. The new degradable food packaging film is safe and effective, with good mechanical properties and excellent fresh-keeping properties, and has a good application prospect in food packaging.