This paper aims to obtain the higher purity of mulberry anthocyanin. Taking mulberry as the research object, the effects of five macroporous resins such as AB-8, D4020, D101, NKA-9 and HPD100 on the adsorption and desorption properties of mulberry anthocyanins were compared, and the optimum process conditions were determined. The results showed that HPD100 macroporous resin was the best for the purification of mulberry anthocyanin, and the adsorption and desorption rates were 93.6% and 37.9%, respectively. The best conditions for purification were:the flow rate of the sample was 1.5 mg/mL, the pH of the sample was 2.4, the volume of ethanol was 100 mL, the elution velocity was 1.5 mg/mL, the ethanol volume fraction was 70%, and the purity was 35.15%. The optimum purification conditions were obtained by using polyamide resin:pH 3.4, ethanol volume fraction 70%, purity of 50.76%. The optimum purification conditions can improve the purity of anthocyanin effectively, and provide important reference for the study of mulberry anthocyanin.