The purpose is to study the applicability of 50% ethanol as the fatty food simulant for food contact rubber. And the results of this study provide data support for the revision of the standard of food contact rubber in China. This paper took the positive rubber samples containing 1,4-diphenyl-1,3-butadiene (DPBD) as the research object. The migration of DPBD from the positive rubber samples to real foods, food simulants (50% ethanol and olive oil) and substitute solvent (95% ethanol) were respectively determined and compared by gas chromatograph-triple quadrupole tandem mass spectrometry (GC-MS/MS). Under the same migration condition, DPBD migration in different simulants and foods showed the rules of 50% ethanol＞ham sausage or luncheon meat and olive oil＞four kinds of foods. For nitrile butadiene rubber (NBR), the migration of DPBD in 95% ethanol was higher than that in olive oil. The results showed that the setting of fatty food simulant (50% ethanol) was not completely applicable in the current standard of food contact rubber. It is recommended to select olive oil as the fatty food simulant. It is not recommended to correct the results of olive oil while the rubber comes into contact with hotpot condiment or butter. It is recommended to correct the results of olive oil while the rubber comes into contact with ham sausage or luncheon meat. It is not recommended to correct the results of 50% ethanol solution while the rubber comes into contact with ham sausage or luncheon meat. 95% ethanol can be used as the substitute solvent for nitrile butadiene rubber (NBR), but it is not recommended for ethylene propylene diene monomer rubber (EPDM).