According to the drop test and simulation analysis of inflatable cushion with different film thicknesses in the market, the influence law of film thickness on cushioning performance of inflatable cushion is obtained to provide a theoretical basis for the selection and use of cushioning materials of inflatable cushion. Cylindrical inflatable cushions have been used as buffer packaging for glass thermos cups to test the acceleration changes during the drop test. The drop test results have been compared with the simulation analysis results. When the drop height is 40 cm, the inflatable cushions with film thicknesses of 55, 60, and 65 μm are used, and the maximum response acceleration of the glass is 246, 283, and 322 m/s2respectively; when the drop height is 60 cm, the maximum response acceleration of the glass is 357, 419, and 452 m/s2respectively; when the drop height is 80 cm, the maximum response acceleration of the glass is 569, 69, and 751 m/s2respectively. Taking the inflatable cushion with a film thickness of 55 μm as an example, when the simulated drop height is 40, 60, and 80 cm respectively, the maximum response acceleration of the glass is 268, 399, and 631 m/s2respectively. The film thickness affects the cushioning performance of inflatable cushion to a certain extent. In a certain range, the smaller the film thickness of inflatable cushion, the better the cushioning performance. At the same time, the simulation results meet the error requirements, which can provide theoretical support for further research of inflatable cushion cushioning materials.