The purpose of this study is to produce reverse osmosis membrane with stable structure and performance by decreasing the reaction rate of interfacial polymerization and controlling the polymerization process. This study proposed a novel buffer layer free interfacial polymerization (BLIP) to adjust the polymerization rate, and investigated the effect of the monomer concentration and reaction time on the structure and performance of BLIP membranes, and then tested the desalination and chlorine resistance of the BLIP membranes by the usage of Na2SO4 and NaCl. When the mass concentration of m-xylylenediamine (m-XDA) and trimesoyl chloride (TMC) were 0.6% and 0.3% respectively, the desalination rates of the BLIP membranes to Na2SO4 and NaCl are the best, which are 92.55% and 83.99% respectively, andthe water flux were 8.30 L/(m2.h) and 9.15 L/(m2.h) respectively. The desalination rates of BLIP membranes to Na2SO4 and NaCl remained above 72% when the membrane was immersed in NaClO solution with active chlorine concentration of 1 g/L for 12 h. The introduction of buffer layer improved the dispersion uniformity of TMC in oil phase, slowed the interface polymerization rate, and significantly enhanced the performance of the BLIP membranes. Therefore, this study proposed a novel, energy saving, simple reverse osmosis membrane preparation process, which provided a new approach for the development of interfacial polymerization process.