The membrane of egg shell has complex fiber network pore structure, which can be used as a template to develop specific structural materials. In order to make full use of waste eggshell, hydrophobic materials were prepared by using eggshell membrane as template. The surface of eggshell membrane was loaded with dimethyl silicone oil and nano PTFE particles by impregnation method, and the hydrophobic material was obtained after drying. The static contact angle was used to characterize the hydrophobicity of the material, and the successful loading of the material was characterized by FTIR and EDS. The apparent structure of the material was tested by SEM and AFM. SEM and AFM tests show that the material has nano scale rough surface, which conforms to the Cassie-Baxter hydrophobic mechanism. The contact angle can reach 147° when the mass ratio of PTFE nano powder to dimethyl silicone oil is 2∶10 and the immersion time is 24 h. Hydrophobic materials can be prepared by eggshell membrane structure. The impregnation method is simple and feasible. The hydrophobic properties of the materials depend on the ratio of components in the impregnation solution and the impregnation time.