The purpose of this experiment is to study the effect of zeolite on the blocking performance of polylactic acid (PLA) film. The method is to mix the mass fractions of 2%, 4%, 6%, 8% and 10% zeolite with PLA by melting, and add epoxy soybean oil to improve the flexibility of the material, and then test the change of zeolite/PLA blow molding film barrier performance with the instrument. The results showed that compared with pure PLA material, the transparency of modified PLA material decreased, crystallization and fog increased, and the oxygen permeability of permeable oxygen was enhanced and then decreased with the increase of zeolite mass fraction. When the zeolite content was 8%, the barrier performance of the composite material was the strongest, the oxygen permeability coefficient was 0.59×10−8 cm3.cm/(cm2.s.Pa), the carbon dioxide permeability coefficient was 1.95×10−8 cm3.cm/(cm2.s.Pa) and the water vapor transmission coefficient was 2.79×10−7 g.m/(m2.h.Pa), all of these are the lowest level. It can be concluded that due to the presence of internal zeolite molecules and the spatial structure formed by it, the diffusion of gas in zeolite modified PLA film is more difficult when the zeolite is added more than a certain amount. Compared with pure PLA film, the composite film (oxygen, carbon dioxide and water vapor) has an excellent gas and moisture barrier effect.