The study is based on the determination of PLA/PBAT-based biodegradable food preservation bags and the migration of the plasticizer acetyl tributyl citrate (ATBC). A gas chromatography-mass spectrometer was used to detect the components in the material and the migration to the food simulant. Based on this, the influence of different temperatures, different thicknesses, different times, and different simulants on the migration regular of ATBC were studied. And FTIR and SEM were used to analyze the changes of the film before and after soaking. Under the conditions of the same temperature and thickness, the migration amount is the largest in ethanol with a volume fraction of 95%; under the same food simulant, the higher the temperature and the longer the time, the greater the migration amount of ATBC; under the same temperature and food simulants, the smaller the thickness, the greater the migration of ATBC. The nature of food simulants, temperature, thickness and time are important factors affecting the migration behavior of ATBC.