The work aims to fabricate a kind of modified film with antifogging and antibacterial properties. Polyglycerol fatty acid esters (PGFE) and master batch (YS) made in laboratory were used as the antifogging additives and calcium propionate (CP) and sodium dehydroacetate (DHA-S) as the antimicrobial additives and then mixed and granulated with PE particles to prepare the antifogging and antibacterial polyethylene active packaging films through extrusion casting with a casting machine. Orthogonal experiments were used to optimize the formulation of the films. The addition of antimicrobial agent and antifogging agent was in mass fraction. Compared with pure PE film (8.64 MPa; mass fraction of 88.46%; elongation at break of 551.72% in longitudinal direction and 589.86% in transverse direction), the films with YS (3%), PGFE (3%), DHA-S (1%) and CP (1%) had better antifogging property, tensile strength (14.58 MPa) and more excellent antimicrobial properties against test bacteria, but had poor light transmittance (82.68%), elongation at break (272.20% in longitudinal direction and 140.20% in transverse direction) and barrier property. Polyethylene films modified by antifogging agent and antimicrobial agent can be used as food packaging materials because of their antimicrobial and antifogging effects and good physical properties.