The work aims to establish a method based on isotope internal standard by ultra performance liquid chromatography-tandem mass spectrometry (UPLC/MS-MS) to enhance the determination ability of bisphenol A (BPA) in canned beer. The canned beer samples were mixed with BPA-13C12 after ultrasonic treatment, and then analyzed by UPLC/MS-MS and quantified by isotope-labelled internal standard. Quantified by the calibration curve of isotope-labelled internal standard, the detection limitation of the method was 1.00 μg/L. The linear equation was y=1.73x+0.206, the correlation coefficient was 0.9992, and the spiked recoveries of BPA was 91.0% to 109% with RSDs ranging from 4.68% to 6.63%. Then, the method was applied to determine the pollutant migration of BPA in 6 canned beers from Xiamen local market. The results showed that BPA was found in all samples with the average concentration of 1.48 μg/L. The method has the advantages of simple and fast pretreatment, low detection limitation, high sensitivity and good accuracy, which can satisfy the requirement of detecting low BPA concentration in canned beer.