The work aims to establish a method for the simultaneous determination of 24 harmful substances (17 PAEs and 7 PCBs) and 7 polychlorobiphenyl (PCBs) in food packaging paper by GC-MS/MS. After sample treatment, 30 mL isopropanol and internal standard solution were added accurately and gas chromatography tandem mass spectrometry was used for detection after ultrasonic extraction for 40 min. Then, the internal standard method was adopted for quantification. In the range of 0.02-1.0 mg/L, the linearity of fitting curves of the 24 compounds was good and the correlation coefficient was in the range of 0.9945-1.0000. The limit of detection and the limit of quantitation were 0.0005-0.0028 and 0.0016-0.0089 μg/mL, respectively. The highest content in 6 kinds of food packaging paper was 26.53 mg/kg. The recovery experiments were carried out to 24 compounds at low, medium and high levels and the average recovery rate was from 85.6% to 109.7%. The relative standard deviations within and between days were less than 6% (n=5) and 9% (n=3), respectively and the recovery rate, repeatability and accuracy were good. This method is simple, rapid, accurate and reliable. It is high-throughput and suitable for the simultaneous determination of 24 kinds of harmful substances in food packaging paper.