The work aims to study the effects of ε-polylysine hydrochloride/chitosan composite film on the preservation of cherries.The cherries were coated with chitosan film and ε-polylysine hydrochloride/chitosan composite film for preservation, respectively, and the uncoated cherries were used as blank control group.Then, the cherries were packaged in disposableperforated fruit box.The changes in main quality factors of the cherries in different groups were studied during cold storage.ε-polylysine hydrochloride/chitosan composite filmshowed the best effects inreducing the weight loss rate, sag rate and rot rate of cherries. After 6 days of storage, the content of titratable acid of the cherry treated with ε-polylysine hydrochloride/chitosan composite film was 0.52%, and the Vc content was 5.95 mg/kg.Compared with the uncoated cherries, the decrease of titratable acid and Vc content in coated cherries was slowed down.ε-polylysine hydrochloride/chitosan composite filmcouldalso improve cherry hardness and color during storage.ε-polylysine hydrochloride can work with chitosan to play a better role in keepingthequalityofcherries.