The paper aims to overview the research progress in preparation of polysaccharide-based antibacterial membrane in recent years so as to provide a scientific basis for further preparation and development of polysaccharide-based antibacterial membrane. Based on analysis and summary of existing research status and results, the types and preparation methods of polysaccharide-based antibacterial membrane substrate were introduced. Physical means such as blending, casting, coating and electrospinning, dissolution-insertion, and chemical cross-linking were the main methods for preparation of polysaccharide-based antibacterial membranes. Physical means are commonly used to make membrane, thus it is urgent to develop natural antibacterial agent. The comprehensive performance of single polysaccharide-based antibacterial membrane is insufficient, its application is easily limited, and the comprehensive performance of composite membrane is good, but the barrier performance and slow release mechanism need to be further studied.