The work aims to study the law of effects of squeegee angle, squeegee speed, off-grid spacing and squeegee pressure on the quality of printed products during screen printing, so as to provide technical reference for the practical application of screen printing electronics. The orthogonal test was carried out on the four printing parameters with the existing screen printing test bench. The partial least squares method was used for the primary and secondary judgment on the influencing factors of the data in the experiment. The off-grid spacing had the most obvious influence on the width and surface roughness of the conductive lines in the printed electronics. The effect of the squeegee pressure, the squeegee angle and the squeegee speed on the surface roughness of conductive lines was not obvious. Based on the orthogonal test analysis, the best technological condition for screen printing was C3A1D1B3. The experimental data were analyzed by partial least squares method to verify the rationality of the orthogonal test. In the screen printing electronic printing process, the correct selection of reasonable off-grid spacing is the key to obtain high quality printed products.