This work aims to study the vibration characteristics and mechanical damage factors of Kiwifruit during transportation, and provide a reliable theoretical basis for enhancing the protection of fruit transportation packaging. The resonance frequency of kiwifruit was detected by sweeping frequency vibration experiment; the fixed frequency vibration experiment of kiwifruit was carried out based on orthogonal experimental analysis method; and the effects of vibration acceleration, packaging buffer material, vibration time and stacking layers on surface damage index and vibration trans-mission rate were analyzed, so as to obtain the sensitive factors on mechanical damage of kiwifruit. The experimental results show that when the vibration acceleration was 0.15g, the resonance frequency of kiwifruit was 85.2 Hz; the damage degree of kiwifruit was the smallest when the paper scraps were used as cushioning material, and the vibration transmission rate was also small. It is concluded that cushioning packaging structure is the most important factor affecting the surface damage index and vibration transmission rate. The cushioning performance of paper scrap liner and EPS liner is better than that of PET liner. The bigger the vibration transmission rate is, the bigger the surface damage index is.