The work aims to explore the optimum extraction conditions of natural raspberry ketone and the anti-lipid peroxidation effect of raspberry ketone. Red raspberry was used as the raw material to extract the raspberry ketone by ul-trasonic-assisted ethanol and the content of raspberry ketone was determined by gas chromatography. Based on the single factor experiment results, the response surface optimization experiment was designed, and the anti-lipid peroxidation effect of raspberry ketone was studied by taking the ratio of material to liquid, ethanol concentration, ultrasonic time and temperature as the influencing factors, and the yield of raspberry ketone as the response value. The optimum extraction conditions of raspberry ketone were as follows: the ratio of material to liquid was 1 : 15 g/mL, the concentration of ethanol was 85%, the ultrasonic time was 50 min, and the ultrasonic temperature was 40 ℃. Under these conditions, the content of raspberry ketone was 2.803 μg/g, and the trend of anti-lipid peroxidation was Vc solution>raspberry ketone crude extract>raspberry ketone standard solution. Ultrasound-assisted ethanol extraction can be effectively applied to extract raspberry ketone from red raspberry, and raspberry ketone crude extract can inhibit lipid peroxidation induced by Fe2+.