The work aims to get the optimal technological conditions for the extraction of lycopene by supercritical CO2. Fresh tomatoes were freeze-dried and crushed, and then single factor and response surface experiments were used to determine the best conditions, which included the extraction pressure, extraction temperature, CO2 flow rate, modifier flow rate and static extraction time. The results showed that static time and extraction temperature had significant effects on the extraction rate of lycopene, and interactions existed between the flow rates of modifier and CO2, between the flow rate of CO2 and the extraction temperature, and between the extraction temperature and the static time. The optimum conditions for the extraction of lycopene by supercritical CO2 were determined. The extraction pressure, flow rates of modifier and CO2, extraction temperature and time were 35 MPa, 5 mL/min, 6 mL/min, 46 ℃ and 90 min, respectively. Under the optimum conditions, the extraction rate of lycopene was up to 90.21% and the lycopene content of tomato powder was 3648.57 μg/g. Under the optimum conditions, the extraction rate of lycopene is higher, the optimization effect is better, and it has considerable practical application value.