The work aims to study the inhibitory activities of four Chinese edible and medicinal herbs against the main pathogenic bacteria of "Guichang" kiwifruit soft rot. The "Guichang" kiwifruit was used as the experimental material to isolate and purify the soft rot of kiwifruit. The inhibitory activity of crude extracts of four kinds of edible and medicinal herbs against kiwi soft rot was tested by inhibiting the growth rate of mycelium. Botrytis cinerea, Phomopsis sp., Botryosphaeria dothidea, and Penicillium sp. were isolated, purified and identified from "Guichang" kiwi soft rot. The four crude extracts of Chinese edible and medicinal herbs had certain inhibitory activities on the four pathogenic bacteria at 50 mg/mL, among which Illicium verum had good inhibitory activity against four pathogenic bacteria. Through mycelial growth inhibition rate method, Illicium verum has good inhibitory effect against Botrytis cinerea, Phomopsis sp., Botryosphaeria dothidea, and Penicillium sp.