The objective is to rapidly detect 2,2-bis (4-glycidyloxyphenyl) propane in lake water. A novel 2,2-bis (4-glycidyloxyphenyl) propane hapten was designed and successfully coupled with carrier protein. Hybridoma cell lines capable of stably secreting BADGE monoclonal antibody were successfully screened by the methods of mouse immuniza-tion, cell fusion technology and three subclones. A high sensitivity and specificity monoclonal antibody against 2,2-bis (4-glycidyloxyphenyl) propane, which can be used for indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) of 2,2-bis (4-glycidyloxyphenyl) propane was prepared successfully. Based on monoclonal antibody, the standard curve was drawn. The results showed that the IC50 of monoclonal antibody was 1.15 ng/mL, and the linear range of IC20-IC80 was 0.16-8.15 ng/mL. The recovery rate of BADGE in lakewater was between 80% and 120%. In conclusion, the application of 2,2-bis (4-glycidyloxyphenyl) propane monoclonal antibody in the detection of lake water is feasible.