The work aims to develop the PBAT/PLA nano-active packaging material with antibacterial function, with polyadipate/butylene terephthalate (PBAT) and polylactic acid (PLA) as substrates, and nano-TiO2 as a functional additive, and study its effect on grape storage period, so as to solve the problems of water loss, mildew and decay in the preservation of fruits and vegetables. The blending melt blow molding process was adopted and the substrate compatibility was controlled to achieve the antibacterial effect of packaging on the surface of fruits and vegetables. The nano-TiO2 degradable packaging film was developed. With the grapes as experimental materials, the packaging film was used for the modified atmosphere storage of the grapes. The effects of packaging films on grape storage quality were studied by measuring the fruit yield, weight loss rate, soluble solid content, total acid content, catalase activity and anthocyanin content. The experimental results showed that, the mechanical properties and breathability and water vapor permeability of the packaging films were good. When the addition amount of nano-TiO2 was 1% (in mass fraction), the fresh-keeping effect of the grape was the best. After 28 days of storage, the grape yield rate was 81.8%, the weight loss rate was 12.8%, the catalase activity was 0.0438 U/(min•g), and the anthocyanin content was 0.87 mg/g. Nano-TiO2 degradable packaging film can significantly reduce grape granules, inhibit grape spoilage, slow down the rate of mildew, maintain high anthocyanin content, and has good preservation effect on grapes.