The work aims to explore the vibration transmissibility properties of the cylindrical air cushion, so as to obtain the vibration transmission rate curves. The sinusoidal frequency sweeping vibration test was carried out on the vibration system formed by cylindrical air cushion and load mass in the frequency range of 3~100 Hz to analyze the influence rule of initial internal pressure and thickness on its vibration transmissibility properties. The results showed that, the initial internal pressure had no significant effect on the vibration isolation capability of the air cushion. The resonance frequencies of the vibration system at three initial internal pressures were all around 35 Hz, and the peak value of the transmission rate was around 11. With the increase of thickness, the resonance frequency and the transmission rate peak were simultaneously reduced, and the vibration isolation capability of the air cushion was enhanced. Therefore, the selection of a suitable initial internal pressure and the use of a multi-layer air cushion can significantly improve its vibration isolation capability while satisfying the cushioning performance. The research results can provide a basic basis for the design of the cushioned vibration isolation package for cylindrical air cushion.