The work aims to prepare the rare earth complex of europium pyridine dicarboxylic acid and add it to offset ink to detect and analyze the fluorescence properties. A photoluminescence rare earth complex was formed by the reaction of rare earth material Eu salt with 2,6-pyridine dicarboxylic acid under certain conditions. The structure of the material was characterized by IR and fluorescence spectra. The synthesized rare earth complex was added to the white offset printing ink, and the ink was printed on the paper by printing suitability instrument. Through fluorescence detection, the main excitation wavelength of the substance was determined to be 365 nm and the emission wavelength was 617 nm. Under the irradiation of an ultraviolet lamp, the ink color of the pattern printed by the ink added with the luminescent material with the mass fraction of 3%~4% was close to the fluorescent color of the rare earth material and the chromaticity of the ink did not change much compared with the previous one under D65 light source, and the printing could be completed under the condition of screening. The rare earth luminous materials are stable in offset printing ink, and the rare earth fluorescent materials can emit light in the ink. The substitution of rare earth luminous ink for organic fluorescent ink is realized, which is beneficial to the protection of the environment.