The work aims to obtain PE food packaging film with excellent antibacterial properties. With polyethylene film (PE) as the substrate, the negatively charged food contact material acrylic resin anionic emulsion (1127-PAA) was coated on PE according to the principle of mutual attraction of static electricity, and then coated with positively charged chitosan (CTS)/lemon oil (LEO) to obtain a PE food packaging film with excellent antibacterial properties. The coating effect was analyzed by amino black staining and contact angle experiment. The effect of the ratio of CTS to LEO in the PE-PAA-CTS/LEO composite film and the number of CTS/LEO layers on the antibacterial property of the composite film was investigated. With chilled pork as a fresh-keeping object, the volatile base nitrogen (TVB-N) value, pH value, total number of colonies, water migration, and color difference (a* value, b* value, L* value), and texture (elasticity, hardness and viscosity) were regularly measured, and the freshness of the chilled pork was analyzed to evaluate the preservation effect of the composite film. PAA and CTS could be uniformly coated on the PE film in sequence. When the contact angle of the film was changed from 71° to 22°, the hydrophilicity was greatly improved. When the ratio of CTS/LEO in the composite film was 1︰1 and the number of CTS/LEO layers was 2, the diameter of the inhibition zone of the Oxford Cup could reach 35.6 mm, which had the best antibacterial property and cost effect. Compared with the pure PE film, based on the total volatile basic nitrogen (TVB-N) value, pH value, total number of colonies, color difference (a* value, b* value, L* value), texture (elasticity, hardness and viscosity) and experimental results of nuclear magnetic resonance index, PE-PAA-CTS/LEO antibacterial can extend the shelf life of chilled pork by 2 days. Compared with the blank PE film, the PE-PAA-CTS/LEO composite film can effectively extend the shelf life of chilled pork by 2 days. When the number of CTS/LEO layers on the PE+PAA film is 2, the bacteriostatic effect and cost are optimal.