The paper aims to detect the problems of collating and design a collating online detection system of the adhesive binding line. The CIS image sensor was used in the system to collect the image of the signature mark, CPLD was applied to control the timing, and a detection algorithm based on column pixel position feature mark was proposed. The image was analyzed and judged by DSP，then the signal was sent to eliminate the wrong book. The experimental results showed that the hardware circuits of the system could be controlled accurately, and each unit could realize the design function in an orderly way. The detection system could judge the collating problem accurately. The design of the detection system takes into account the use and installation environment of adhesive binding Line, and has obvious advantages in cost of installation and use. It can be used for detection of collating.