The paper aims to study the effects of different controlled atmosphere treatments on storage quality and related enzymes activities of Huangguan Pear, and to screen out better controlled atmosphere storage conditions. Under the conditions of temperature (0±1) ℃ and relative humidity 90%~95%, Huangguan Pear was used as the research material, and it was treated with three different O2 components (O2(1%~2%)+CO2(1%~2%), O2(3%~4%)+CO2(1%~2%), O2(5%~6%)+CO2(1%~2%)), the changes of pear fruit quality and enzyme activity were observed. Compared with the control group, the three different O2 and CO2 combinations of controlled atmosphere storage reduced the browning rate, respiratory intensity and ethylene release of the Huangguan Pear, maintained a higher fruit rate and peel color, inhibited fruit softening and PPO (Polyphenol Oxidase) activity in the core, and maintained a higher CAT(Catalase) activity, thus inhibited pear browning. Especially, the treatment combination 2(O2(3%~4%)+CO2(1%~2%)) had the most significant effect. Controlled atmosphere storage maintainshigh storage quality of Huangguan Pear and inhibits browning. The treatment combination2 (O2(3%~4%)+CO2(1%~2%)) is the optimal controlled atmosphere storage of Huangguan Pear.