The work aims to prepare an environment friendly water-based ink cleaner suitable for offset printing press. Tween 80 and dodecyl dimethyl betaine were used as surfactants and sodium carbonate as an alkaline auxiliary to achieve cleaning effect, and deionized water and alcohol were used as solvent, triethanolamine as anti-redeposition, benzo Triazole as rust inhibitor, urea as solubilizer and sodium chloride as electrolyte to study the synthesis conditions of environment friendly ink cleaner. The factors affecting the product properties were investigated by univariate variable method. The optimal formula was obtained: 70 g water, 15 g alcohol, 6 g dodecyl dimethyl betaine, 12 g Tween 80, 3 g sodium carbonate, 2 g urea, 1 g benzo Triazole, 1 g triethanolamine, appropriate amount of sodium chloride and defoamer. The product which was synthesized under this condition was transparent and clarified with cleaning rate of 99.8%. The comprehensive performance of the product is good and the product is suitable for cleaning the ink of the offset printing machine.