This research aims to compare permeability and flex resistance of different packaging materials, study the rationality of materials in different packaging for food and discuss the comparability and applicability of materials with different permeability through differential-pressure method and equal-pressure method. Oxygen permeability of samples including PET/Al/PA/CPP, PET/Al/CPP, PET/VMPET/CPP, PET/VMCPP, PET/PET-AlOx/CPP, KPET/CPP, PET- AlOx/PA/CPP, KOPP/CPP, PET/CPP, BOPP/CPP were tested with differential-pressure method and equal-pressure method before and after 270 cycles of flex test. The oxygen permeability rate of the samples before flex tests from high to low were PET/Al/PA/CPP＞PET/Al/CPP＞PET/VMPET/CPP＞PET-AlOx/PA/CPP＞PET/PET-AlOx/CPP＞PET/VMCPP＞ KOPP/CPP＞KPET/CPP＞PET/CPP＞BOPP/CPP. Compared with their oxygen permeability rate before flex tests, the oxygen permeability rate of KOPP/CPP, KPET/CPP, PET/CPP and BOPP/CPP after flex tests were increased by less than 3%, while for the other six samples, the oxygen permeability rate were increased by at least 5 times. The test results of all samples except PET/Al/PA/CPP obtained with both differential-pressure method and equal-pressure method were within the deviation of ±10%. Due to the differences in material structure, types of coatings, thickness and flexibility, the permeability and flex resistance of the ten samples are quite different, for which, they are suitable for different food packaging. In addition, though differential-pressure method gives a slightly higher result when testing high barrier materials; equal-pressure method gives a lower result when testing high permeability materials. Both methods are applicable to determine the oxygen permeability of all the samples except PET/Al/PA/CPP.