The work aims to take Jufeng grape as the test object to study the effects on the physiological quality caused by drop impact, mechanical damage and vibration fatigue accumulation during storage and transportation. Grapes were tested when dropped from different heights under the existing packaging conditions to obtain the effects of dropping height on grape mechanical properties. At the same height, the tests were performed again with the new damping package to obtain effects of new cushioning packages on grape mechanical properties. Afterwards the simulated transport vibration test bench and the six-degree space vibration test bench were used to simulate the effects of different packages on grape physiological quality under actual transportation conditions. From the study, the weight loss rate of the new vibration-damping package under two vibration and fatigue accumulation tests was reduced by 27.07% and 21.42% compared to the ordinary packaging, and the surface damage coefficient decreased by 20.11% and 17.61%. The studies can provide a theoretical basis for designing reasonable packaging and transportation methods.