The work aims to detect zearalenone in edible packaging materials, and provide effective technical means for real-time monitoring of food packaging and packaging storage conditions, etc., mainly through enzyme-linked immunosorbent assays, supported by colloidal gold test strips. Corn flour (edible packaging material) was mainly selected as the experimental detection object. Through the addition recovery experiments, the maximum recovery rate was used to obtain the optimal pretreatment conditions, thereby improving the accuracy of the detection results of enzyme-linked immunosorbent assays. After optimizing the closed temperature and incubation time of the standard curve, the pretreatment method of zearalenone (including optimizing the concentration of the extractive solution, the pH value of the dilution and the concentration of organic solvent in the dilution, with the addition recovery result as the judgment basis) was tested. Meanwhile, the optimized experimental results were assembled into the colloidal gold test strips, and the zearalenone-containing corn flour was quickly detected. Through experiments, it was determined that the optimal conditions for the pretreatment were 50% (volume fraction) methanol solution, and buffer solution at pH 8.0 and with 10% (volume fraction) methanol. The recovery rate measured under this condition was the highest. In such optimal conditions, the detection limit of the assembled colloidal gold test strip could reach 0.75 ng/mL. The detection results are basically the same as the experimental results measured by addition level. It can be confirmed that the colloidal gold test strip is an effective rapid detection method.