The work aims to design a cold storage phase-change incubator with two temperature zones in order to reduce the loss of cooling capacity of the incubator and store a variety of fresh foods together. The incubator has a cold storage zone and a superchilling storage zone, and the consumption of the cold storage material in each zone was obtained by thermal load calculation. The heat insulation effect of the incubator was studied by numerical simulation and experimental verification. Finally, the test data were compared with the simulated data to verify the reliability of theoretical calculation and numerical simulation. When the cold storage plate was placed at the top of the incubator, the temperature in the middle of the incubator was the highest, and the temperature at the top was the lowest. Although the temperature field was sometimes unevenly distributed, the overall temperature of the two zones tended to be stable within 2 h, and maintained at 1~4 ℃ and ?10~0.5 ℃, respectively, which were suitable for fresh foods preservation. The designed incubator can effectively maintain the preservation temperature and meet the design requirements of various fresh foods stored together.