The work aims to explore the effect of different models on the temperature field simulation results of the incubator under no-load conditions. The actual temperature field in the existing incubator was measured and comparatively analyzed with the established finite element model of the incubator to verify the reliability of the model. Based on that, the effects of boundary conditions, radiation model and convection model on the simulation results of temperature field in the incubator were further explored. By verifying mesh independence, the optimal fluid mesh size obtained was 2.8 mm. The constant temperature solid wall and the convective heat transfer wall were used by the boundary conditions to obtain the maximum temperature difference between the temperature field simulation results, which was 0.1 K. The maximum temperature difference between the simulation results and the experimental results obtained by P1 radiation, Rosseland, DO radiation and non-radiation model was 1.1, 4.2, 4.3, 4.3 K, respectively. The maximum temperature difference between the simulation results and the experimental results obtained by the laminar models and the turbulence models was 0.6 K and 1.9 K, respectively. With the decrease of mesh size, the simulated data of temperature field gradu-ally become stable. The simulation results of temperature field obtained by the boundary conditions using the constant temperature solid wall and the convective heat transfer wall are basically the same. The simulation results of P1 radiation model agree well with the experimental results by comparing with P1, Rosseland, DO and non-radiation models. Compared with the turbulence model, the simulation results obtained from the laminar model agree well with the experimental results.