The work aims to study the influence of TiO2 antibacterial agents of different kinds and different mass fractions on the antibacterial effect and physical and mechanical properties of the packaging paper. The nano-TiO2 antibacterial agent suspension was coated on the surface of the antibacterial paper for drying treatment. Then, the antibacterial properties and physical and mechanical properties were tested. The effect of nano-TiO2 on antibacterial properties and physical and mechanical properties of antibacterial paper was studied. Nano-TiO2 antibacterial paper had inhibitory effect on staphylococcus aureus and escherichia coli. The concentration values of different kinds of TiO2 antibacterial paper were different when they reached the best antibacterial effect. In addition, nano-TiO2 antibacterial agent had no significant effect on whiteness, folding resistance, tear strength and bursting strength of paper. On the basis of maintaining its original physical and mechanical properties, the nano-TiO2 antibacterial paper can effectively inhibit the propagation and growth of bacteria, and can be widely used as packaging paper with antibacterial effect.