The work aims to study the effects of treatment by simple spontaneous modified atmosphere packaging (S-MAP) and methyl jasmonate (MeJA) of different concentrations on the postharvest blueberry fruit resistance. Blueberry fruit was immersed in 0.15 and 0.3 mmol/L MeJA and deionized water for 10 min, respectively; then, the fruit was air-dried, precooled, filled into polyethylene S-MAP with a thickness of 0.0294 mm and stored at 1 ℃. The indexes of respiratory metabolism and resistance of blueberry were determined in a period of 15 d, including the concentration of CO2 and O2 in the S-MAP, fruit brightness, sensory evaluation, respiration rate, firmness, decay rate, pH, soluble solid content (SSC), phenylalanine ammonia-lyase (PAL) activity, polyphenol oxidase (PPO) activity, peroxidase (POD) activity and lignin content. MeJA treatment could effectively maintain the appropriate concentration of CO2 and O2 in the bag, slow down the decline of fruit firmness and brightness, inhibit the rise of respiration rate, pH value and SSC, increase the lignin content, reduce the decay rate, and significantly improve the activities (P<0.05) of enzymes related to the resistance, such as PAL, PPO and POD. S-MAP combined with MeJA treatments can induce the generation of the fruit resistance and maintain the quality of postharvest fruits by inhibiting the respiratory metabolism, and increasing the metabolism of phenylpropane and the activity of antioxidant enzyme. During the MeJA treatments of two concentrations, the treatment effect is the best when theh concentration is 0.15 mmol/L.