The work aims to study the oxidation resistance and fresh-keeping performance of the biodegradable polylactic acid (PLA)/poly(butylenesuccinate adipate) (PBSA) film added with organic nano montmorillonite (MMT) and clove essential oil. PLA/PBSA/MMT/essential oil nanocomposite films were prepared through extrusion blending modification and extrusion casting method. The mechanical property, moisture permeability, air permeability and oxidation resistance, etc. of the films were discussed; moreover, the preservation effect of cherries was studied. The PLA/PBSA film added with DK4 MMT not only increased its tensile strength, but also decreased the WVP and OP. However, there was no significant difference from the control indexes in the preservation of cherries. On this basis, after the addition of clove essential oil, mechanical property was improved, the antioxidation was obvious and the preservative effect of cherries was significantly improved. PLA/PBSA film added with DK4 MMT and clove essential oil has better modification effect and good preservative effect.